Generation of multicolored, prestained molecular weight markers for gel electrophoresis.
نویسندگان
چکیده
Molecular weight marker proteins are routinely used in sodium dodecyl sulfate-polyacrylamide gel electrophoresis to estimate the relative molecular mass of specific proteins within a sample. This report describes a simple procedure for the generation of multicolored molecular weight proteins using a variety of Remazol-reactive textile dyes. These multicolored proteins provide a set of unambiguous markers for gel electrophoresis. Furthermore, the colored markers can be used in conjunction with Western blotting techniques to provide a visual display of marker proteins on the transfer membrane.
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ورودعنوان ژورنال:
- Electrophoresis
دوره 23 19 شماره
صفحات -
تاریخ انتشار 2002